Identification of a C2H2 Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in Phytophthora sojae Based on WGCNA

Identification of a C2H2 Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in Phytophthora sojae Based on WGCNA

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Phytophthora sojae is a destructive soybean pathogen that orchestrates various secreted proteins (effectors) to modulate plant immunity and facilitate infection.Although a number of effectors have been identified and functionally studied in P.sojae, the way these molecules are regulated is marginally known.

In this study, we performed a weighted gene correlation network analysis (WGCNA) based on digital RNA-seq, which enabled the identification of a transcription factor (PsCZF3) in P.sojae.This transcription Hair Styling Tools factor is a C2H2-type zinc finger protein that regulates the transcription of 35 RxLR effectors during the early infection stage.

Phylogenetic Latigo analysis revealed that PsCZF3 is a highly conserved protein across oomycetes, suggesting that this regulation mechanism may broadly exist in oomycete species.In addition, by building a subnetwork of PsCZF3 and correlated genes, we also found that PsCZF3 contributed to the transcriptional regulation of carbohydrate-active enzymes.Our findings suggest that the activation of PsCZF3 facilitates P.

sojae infection by up-regulating RxLR effectors and carbohydrate-active enzymes.